Background/Objectives: Clostridioides difficile is a “One Health” pathogen and a cause of antibiotics-associated diarrhea and pseudomembranous colitis. Mobile genetic elements (MGEs) have been documented in the genomes of clinical C. difficile strains; however, the presence of MGEs in environmental strains remains poorly characterized. Thus, the present study was conducted with the objective of identifying the prevalence of MGEs, including mobilizable transposons (MTns), conjugative transposons (CTns), plasmids, and insertion sequences, in whole genome sequences (WGSs) of environmental C. difficile isolates. Methods: The analysis of MGEs was conducted using 166 WGSs obtained from C. difficile strains isolated from various environmental sources contaminated with feces. The MGEs were identified using bioinformatic tools. Results: A total of 48.2% (80/166) of the studied genomes were identified to harbor nine transposons, including Tn916, Tn6194-like, Tn5397, Tn6215, Tn4001, Tn6073, Tn6110, Tn6107, or Tn5801-like. The majority of MTns and CTns could be found within C. difficile sequence types ST11, ST3, and ST35. The results demonstrated close genetic relatedness among the studied genomes, the array of antimicrobial resistance (AMR) genes, such as tetM, ermB, and aac(6′)-aph(2″), and the presence of CTns. Furthermore, the analysis revealed that 24.7% (41/166) of the genome sequences of isolates were associated with various predominant plasmid groups, including pCD6, pCD-ECE4-6, pCD-WTSI1-4, pCDBI1, and pCd1_3, which belonged to 16 different sequence types. Furthermore, several plasmids were identified as harboring the prophage phiCDHM19. Conclusions: The results of the current study suggest that the identified plasmids are abundant and may encode functions that are relevant to C. difficile physiology. The genomes of C. difficile strains examined contain closely related CTns, suggesting that horizontal transfer of AMR is important in this species or other bacterial species. Further research is required to ascertain the effect of these genetic elements and their transferability on the biology of C. difficile.

Abstract. The cosmopolitan or banded garden spider, Argiope trifasciata (Forskål, 1775), the lobed spider, A. lobata (Pallas, 1772) and the MENA lobed spider, A. sector (Forsskål, 1776) are reported, figured and described for the first time in Gharyan city, northwestern of Libya, based on adult female specimens gathered from different plants:- amaranth, haloxylon, prickly saltwort, retama, safflower, vetches, across various sites in the city. Unpublished reports of Argiope species were recorded in 1934 along the Libyan coast.

Abstract The integration of Artificial Intelligence (AI) in education is progressively transforming teaching, learning, and administrative practices globally, with significant potential for application in Libya. the purpose of this research is to discuss and evaluate the benefits and risks of AI in the education system of Libya. To achieve this, a descriptive research approach was employed, using a questionnaire distributed to a selected group of educational professionals in Libya. The sample consisted of 1,000 participants, and the survey was conducted via the WhatsApp application. The results highlight that personalized learning received the highest mean score of 4.19, indicating strong agreement on its effectiveness. Automation of administrative tasks (mean 3.80) and data-driven decision-making (mean 3.98) also showed favorable perceptions, with low variances around 0.24 0.25, reflecting consistent responses. Improved student engagement had a mean of 3.91 and a standard deviation of 0.49, similar to others, suggesting overall positive and uniform attitudes. Additionally, most responses clustered around neutral to moderate agreement, with modes of 3 or 4, showing balanced or mildly supportive opinions. Variance values ranging from 1.39 to 1.48 suggest a moderate spread, indicating some disagreement among participants. Standard deviations around 1.18 to 1.21 confirm a consistent yet moderate level of variability, without extreme polarization The obtained results show strong agreement on the benefits of educational innovations, especially personalized learning, which had the highest mean, and all aspects scored highly with low variability, indicating consistent positive perceptions. At the same time, the conclusions reveal moderate concern among respondents about the cost and inequality issues related to AI in education, with variance and standard deviation values reflecting a consistent spread of opinions. This research contributes to the academic discourse on the impactful integration of AI in academic institutions and establishes positive perceptions for continual enhancement. Overall, funding infrastructure emerges as the most pressing challenge, with general agreement across participants. Keywords. Artificial Intelligence, Education, Personalized Learning, Libya. 

SUMMARY This research documents the initial finding of Chloris pycnothrix Trin., frequently referred to as spiderweb chloris, in Libya, particularly from the Ariggiba region close to Sabha city. This grass, originating from tropical and subtropical regions, is versatile and may become invasive. It was recognized via morphological examination during field surveys in 2023–2024, with samples stored in a herbarium. The species flourishes in alluvial plains as well as in disturbed habitats like road shoulders. It can be mistaken for Chloris virgata, although they differ in some important morphological aspects. It was previously believed that only one species of Chloris existed in Libya. This study provides a revision and an identification key for them in the context of Libyan flora. The tolerant nature of C. pycnothrix to drought, combined with its ability to colonize poorly maintained land, makes its potential introduction—most likely through anthropogenic means—alarming ecologically and emphasizes the need for management strategies to contain its spread and monitor its impact. 

ABSTRACT

Argemone ochroleuca Sweet, Brit. Fl. Gard. Ser.1 3(2): pl. 242 (1828) of the family Papaveraceae is reported here for the first time from Libya. This species is native to Mexico. Flowering specimens of A. ochroleuca were collected from the region Ariggiba (26°35’11.8”N 13°29’24.8”E), 110 km southwest of the city of Sabha, which lies about 700 km south of Tripoli. It is presented with updated nomenclature, taxonomic description, geographical distribution, key to genera, location in Libya, and colour photographs taken in the field. The voucher specimens are deposited in the Herbarium of Botany Department (ULT), University of Tripoli, Libya.

We present a solution method for a general Riccati differential equation by imposing relationships between

the coefficients of the Riccati differential equation and explaining them through proofs and examples, we can find the

general solution to the various forms of Riccati's equation by integration directly after transforming it into a separable

differential equation.

A new record for Leptadenia arborea (Forssk.) Schweinf. is reported for the first time in the flora of Libya. This species was collected from Ariggiba region (110 km southwest Sabha city). A full description and habitat information on the plant are provided. A brief discussion about the most important traits of this species is presented.

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:Abstract

Smoking causes about 6 million deaths annually, increases the risk for many diseases and alters hematological parameters. Thus, the aim of this study was to evaluate the negative impact of cigarette smoking on the hematological parameters of healthy smokers in Libya. A total of 145 smokers and 145 non-smokers, with age range 39-45 years, were recruited. Blood samples were collected from each participant and were analysed for complete blood count. The obtained results were statically analysed using descriptive statistics and ANOVA. The mean for all study variables for smokers (except for MCHC) was greater than that for nonsmokers. The coefficient of variation (CV%) showed that the smokers group was more homogeneous for most variables than the non-smokers group, except for BMI and LYMP. The results of ANOVA test showed that only in the case of MCHC the non-smokers had a significantly higher mean (p < 0.05). For other variables, the smokers had significantly higher means (p < 0.05), except for the RBC, where the mean was non-significantly higher compared to the non-smokers. There was no statistically significant effect for the age variable or for the interaction between smoking and age on all variables. In conclusion, the study revealed that cigarette smoking has a significant impact on most haematological parameters; confirming that smoking has severe adverse effects on most haematological parameters

Abstract

For the first time in Libyan flora, a new record for Boerhavia repens L is recorded. This widespread wild weed was collected from the Ariggiba region (110 km west-south Sabha city). A full description, habitat information, distribution map, and distribution data are provided to facilitate further identification and future detection. A brief discussion about the most important threats this species poses is presented.